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One limitation of HILIC chromatography is the poor retention of neutral (non-polar) lipids such as mono/di/triacylglycerols, cholesterol, free fatty acids, etc. These neutral lipids tend to co-elute very early close to the solvent front (i.e., void volume peak) during HILIC. Thus C30-RPLC was chosen for analyzing neutral lipids as a complementary approach to HILIC. C30-RPLC has been compared with other reverse phase chromatographic methods (C8 and C18) and has been demonstrated to be very efficient in separating neutral lipids, especially triacylglycerols with high selectivity and geometric peak shapes24,25,28. In addition, the combination of C30RP with high resolution accurate mass spectrometry offer superior capabilities in separating neutral lipid isobars and isomers present in biological samples. We applied the C30-RPLC approach to separate the neutral lipids present in our standard mix using positive ion mode as shown in Fig. 7.
The possibility of having several layers with different domain structures on top of each other has not been specifically addressed theoretically even though this possibility was reported for tetragonal PZT thin films [121]. In addition, the case of 3-domain structures has only been considered under stress-free conditions to explain experimental results [122].
The misfit dislocations, through the localized strain field generated around them, induce locally a strong reduction of polarization which is detrimental to the properties of the film (see [174] and references therein for the consequences on polarization loops, dielectric, and piezoelectric properties). This was confirmed recently by the study of polarization and piezoelectric properties on Pb(Zr0.52Ti0.48)O3 thin films [110]. The strong reduction of polarization was also shown recently to reach 48% in a Pb(Zr0.2Ti0.8)O3 film deposited on SrTiO3 and covered by a SrTiO3 film [181]. Morelli et al. [182] attributed this effect to the steps observed in the domain walls [71] giving rise to leakage currents that dominate the polarization reversal measured by piezo-force miscroscopy. Interfacial dislocations were also held responsible for the poor piezoresponse observed for Pb(Zr0.52Ti0.48)O3 nanoislands [183]. A comparison between the properties of Pb(Zr0.2Ti0.8)O3 films free from extended defects and films presenting 90 domains have been carried out by Vrejoiu et al. [163]. If the polarization is indeed reduced in the case of defective films, the dielectric constant was found to be higher, indicating that the pinning of the domain walls by misfit dislocations is not as effective as to suppress completely extrinsic contributions to the dielectric properties of ferroelectric thin films as predicted in [174]. A recent review of both the influence of growth conditions and of the microstructure of perovskite films has been published recently by Vrejoiu et al. [184]. 153554b96e
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